![]() ResultsĪfter screening, 88,548 high-quality cells were obtained and identified. Cell proliferation, wound healing and clone formation experiments explored the function of differentially expressed gene BMPR1B, which were confirmed by tumor models in vivo. Pseudotime, cell–cell communication and pathway analysis were performed to characterize the zone-specific microenvironment. Through scRNA-seq data analysis, we compared the cell types and their transcriptional characteristics in the different zones. ![]() ![]() Methodsįor in vitro studies, single-cell RNA sequencing (scRNA-seq) was used to characterize the cells from the tumor zone, the normal zone and the interface zone with 5-mm-wide belts between the tumor invasion front and the normal zone. However, the heterogeneity and characteristics of the microenvironment in the interface area have not yet been thoroughly explored. The interface zone, area around invasive carcinoma, can be thought of as the actual tissue of the tumor microenvironment with precedent alterations for tumor invasion.
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